Anti-Rac1-GTP Monoclonal Antibody丨如何使用
发布时间:2023-01-04
*本公司销售的所有产品仅供实验科研使用,不用于人体及临床诊断。 近期相关文献: |
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货号: 26903 |
抗原: Full Recombinant Active Rac |
规格: 30 微升 |
产品全名: Anti-Rac1-GTP Monoclonal Antibody |
应用: IP, IHC, IF |
种属反应性的选择: Human, Mouse and Rat |
物种/宿主: Mouse |
背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
免疫原: Recombinant full length protein of active Rac1 |
免疫沉淀/蛋白质印迹: Rac Activation Assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel). |
免疫荧光: Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000. |
NewEast Biosciences十二年前率先研发俩种独特的抗体,这俩种抗体仅仅识别活性的GTP酶或者突变的Oncogene
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- 武汉费斯德生物科技公司-NewEast Biosciences 2023-01-04